DEVELOPMENT OF LOW-COST CULTURE MEDIUM USING AGRO-INDUSTRIAL WASTE.

Abstract

The inputs used in microbiology are purchased from companies that use expensive components and very elaborate ways of making the media, making them more expensive. In this sense, the objective of this work was to develop an alternative culture medium from agro-industrial waste with low production cost. For the preparation of the culture medium, pineapple crown and cassava peel were used, which after being dried and properly pulverized were used as a base in the production of alternative culture media for microbial growth. For this purpose, solid and liquid culture media made from the residue flour (30 g/L) and agar (15 g/L) added with salt concentrate (NaNO3 0.08 g/L, MgSO4. 7H2O 0.02 g/L, K2HPO4 0.03 g/L and KCl 0.01 g/L) were tested. After testing bacteria and fungi, it was found that the media presented the necessary characteristics for the growth of Candida albicans and Candida parapsilosis and the bacteria Streptomyces sp. in solid and liquid formulations. Thus, waste-based culture media are a viable alternative for adoption in classrooms, since they have a low cost and an accessible manufacturing methodology so that teachers can implement this technique within the school environment.