MODULATING ACTION OF AROMATIC PLANTS AND PROBIOTICS ON ENZYME ACTIVITY

Aromatic plants and probiotics acting on enzymes

Resumo

The present study aimed to evaluate the effects of extracts from aromatic plants and probiotics on the enzymatic activities induced by Bothrops moojeni venom, as well as to assess their potential as anti-inflammatory agents. The venom’s phospholipase and hemolysis activities were found to be inhibited by incubation with pure plant extracts or probiotics at different pH levels. Additionally, all plant extracts with and without probiotics displayed an inhibitory effect on heat-induced hemolysis and venom-induced thrombus lysis. The greatest inhibitions were observed for extracts of Laurus nobilis and Ocimum basilicum. Furthermore, the extracts of L. nobilis, O. basilicum and Origanum vulgare with and without probiotics were found to exert an inhibitory action on coagulant enzymes present in the venom, prolonging the clotting time. Interestingly, the Petroselium crispum extract in the presence of the probiotic appeared to potentiate the coagulant effect of the venom’s enzymes, as observed by a reduction in the clotting time. The findings demonstrate the potential of these plants to modulate the action of enzymes that play a crucial role in the regulation of hemostasis and the inflammatory response, providing a valuable laboratory tool for the study of compounds with pharmaceutical potential.